DNK polimeraza beta

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DNK polimeraza beta

PDB prikaz baziran na 1bno.

Dostupne strukture

1BPX, 1BPY, 1BPZ, 1MQ2, 1MQ3, 1TV9, 1TVA, 1ZJM, 1ZJN, 1ZQA, 1ZQB, 1ZQC, 1ZQD, 1ZQE, 1ZQF, 1ZQG, 1ZQH, 1ZQI, 1ZQJ, 1ZQK, 1ZQL, 1ZQM, 1ZQN, 1ZQO, 1ZQP, 1ZQQ, 1ZQR, 1ZQS, 1ZQT, 2FMP, 2FMQ, 2FMS, 2I9G, 2ISO, 2ISP, 2P66, 2PXI, 3C2K, 3C2L, 3C2M, 3GDX, 3ISB, 3ISC, 3ISD, 3JPN, 3JPO, 3JPP, 3JPQ, 3JPR, 3JPS, 3JPT, 3LK9, 3MBY, 3OGU, 3RH4, 3RH5, 3RH6, 3RJE, 3RJF, 3RJG, 3RJH, 3RJI, 3RJJ, 3RJK, 3TFR, 3TFS, 7ICE, 7ICF, 7ICG, 7ICH, 7ICI, 7ICJ, 7ICK, 7ICL, 7ICM, 7ICN, 7ICO, 7ICP, 7ICQ, 7ICR, 7ICS, 7ICT, 7ICU, 7ICV, 8ICA, 8ICB, 8ICC, 8ICE, 8ICF, 8ICG, 8ICH, 8ICI, 8ICJ, 8ICK, 8ICL, 8ICM, 8ICN, 8ICO, 8ICP, 8ICQ, 8ICR, 8ICS, 8ICT, 8ICU, 8ICV, 8ICW, 8ICX, 8ICY, 8ICZ, 9ICA, 9ICB, 9ICC, 9ICE, 9ICF, 9ICG, 9ICH, 9ICI, 9ICJ, 9ICK, 9ICL, 9ICM, 9ICN, 9ICO, 9ICP, 9ICQ, 9ICR, 9ICS, 9ICT, 9ICU, 9ICV, 9ICW, 9ICX, 9ICY

Identifikatori

Simboli

POLB; MGC125976

Vanjski ID

OMIM: 174760 MGI: 97740 HomoloGene: 2013 GeneCards: POLB Gene

EC broj

2.7.7.7

Ontologija gena
Molekularna funkcija	• vezivanje oštećene DNK • aktivnost DNK-usmerene DNK polimeraze • proteinsko vezivanje • vezivanje mikrotubula • aktivnost transferaze • aktivnosti nukleotidiltransferaze • aktivnost liaze • vezivanje enzima • vezivanje metalnog jona
Celularna komponenta	• nukleus • nukleoplazma • citoplazma • mikrotubula • vreteno mikrotubule
Biološki proces	• DNK-zavisna DNK replikacija • DNK popravka • popravka ekscizije baza • popravka pirimidinskog dimera • popravka nukleotidne ekscizije, popunjavanje DNK praznina • anti apoptoza • respons na DNK oštećenja • ćelijska smrt • respons na etanol

Pregled RNK izražavanja

podaci

Ortolozi

Vrsta

Čovek

Miš

Entrez

5423

18970

Ensembl

ENSG00000070501

ENSMUSG00000031536

UniProt

P06746

Q8K409

RefSeq (mRNA)

NM_002690.2

NM_011130.2

RefSeq (protein)

NP_002681.1

NP_035260.1

Lokacija (UCSC)

Chr 8:
42.2 - 42.23 Mb

Chr 8:
23.74 - 23.76 Mb

PubMed pretraga

[1]

[2]

Regulatorni element u POLB

Predviđena sekundarna struktura stem loopII (M2) regulatornog elementa POLB
Identifikatori
Simbol	POLB
Rfam	RF01455
Entrez	5423
HUGO	POLB
OMIM	174760
RefSeq	NM_002690
Drugi podaci
RNK tip	Cis-reg
Domain(i)	Mammalia
Lokus	Hromozom 8 p11.2

DNK polimeraza beta (POLB) je enzim koji kod ljudi kodira POLB gen.^[1]

Funkcija

U eukariotskim ćelijama, DNK polimeraza beta (POLB) izvodi popravke ekscizijom baza (BER) koje su neophodne za popravku DNK, replikaciju, rekombinaciju, i otpornost na lekove.^[1]

Regulacija ekspresije

DNK polimeraza beta održava genomski integritet putem uzimanja učešća u popravci ekscizijom baza. Prekomerno izražavanje POLB iRNK je u korelaciji sa brojnim tipovima kancera, dok deficijencije POLB-a rezultuju u hipersenzitivnosti na alkilirajuće agense, uključujući apoptozu, i prekid hromozoma.^[2] Iz tih razloga, esencijalno je precizno održavanje kontrole POLB ekspresije.^[3]^[4]^[5]^[6]

Interakcije

DNA polimeraza beta formira interakcije sa PNKP^[7] i XRCC1.^[8]^[9]^[10]^[11]

Reference

↑ ^1,0 ^1,1 „Entrez Gene: POLB polymerase (DNA directed), beta”.
↑ Narayan S, He F, Wilson SH (August 1996). „Activation of the human DNA polymerase beta promoter by a DNA-alkylating agent through induced phosphorylation of cAMP response element-binding protein-1”. J. Biol. Chem. 271 (31): 18508–13. DOI:10.1074/jbc.271.31.18508. PMID 8702497.
↑ Canitrot Y, Cazaux C, Fréchet M i dr.. (October 1998). „Overexpression of DNA polymerase beta in cell results in a mutator phenotype and a decreased sensitivity to anticancer drugs”. Proc. Natl. Acad. Sci. U.S.A. 95 (21): 12586–90. DOI:10.1073/pnas.95.21.12586. PMC 22874. PMID 9770529.
↑ Bergoglio V, Pillaire MJ, Lacroix-Triki M i dr.. (June 2002). „Deregulated DNA polymerase beta induces chromosome instability and tumorigenesis”. Cancer Res. 62 (12): 3511–4. PMID 12067997.
↑ Bergoglio V, Canitrot Y, Hogarth L i dr.. (September 2001). „Enhanced expression and activity of DNA polymerase beta in human ovarian tumor cells: impact on sensitivity towards antitumor agents”. Oncogene 20 (43): 6181–7. DOI:10.1038/sj.onc.1204743. PMID 11593426.
↑ Srivastava DK, Husain I, Arteaga CL, Wilson SH (June 1999). „DNA polymerase beta expression differences in selected human tumors and cell lines”. Carcinogenesis 20 (6): 1049–54. DOI:10.1093/carcin/20.6.1049. PMID 10357787.
↑ Whitehouse, C J; Taylor R M, Thistlethwaite A, Zhang H, Karimi-Busheri F, Lasko D D, Weinfeld M, Caldecott K W (January 2001). „XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair”. Cell (United States) 104 (1): 107–17. DOI:10.1016/S0092-8674(01)00195-7. ISSN 0092-8674. PMID 11163244.
↑ Wang, Liming; Bhattacharyya Nandan, Chelsea Diane M, Escobar Pedro F, Banerjee Sipra (November 2004). „A novel nuclear protein, MGC5306 interacts with DNA polymerase beta and has a potential role in cellular phenotype”. Cancer Res. (United States) 64 (21): 7673–7. DOI:10.1158/0008-5472.CAN-04-2801. ISSN 0008-5472. PMID 15520167.
↑ Fan, Jinshui; Otterlei Marit, Wong Heng-Kuan, Tomkinson Alan E, Wilson David M (2004). „XRCC1 co-localizes and physically interacts with PCNA”. Nucleic Acids Res. (England) 32 (7): 2193–201. DOI:10.1093/nar/gkh556. PMC 407833. PMID 15107487.
↑ Kubota, Y; Nash R A, Klungland A, Schär P, Barnes D E, Lindahl T (December 1996). „Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein”. EMBO J. (ENGLAND) 15 (23): 6662–70. ISSN 0261-4189. PMC 452490. PMID 8978692.
↑ Bhattacharyya, N; Banerjee S (July 2001). „A novel role of XRCC1 in the functions of a DNA polymerase beta variant”. Biochemistry (United States) 40 (30): 9005–13. DOI:10.1021/bi0028789. ISSN 0006-2960. PMID 11467963.

Literatura

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