Reverzna transkriptaza telomeraze

TERT
Available structures
PDBPretraga ortologa: PDBe RCSB
List of PDB id codes

2BCK, 4B18, 4MNQ

Identifikatori
AlijasiTERT
Spoljašnji IDOMIM: 187270 MGI: 1202709 HomoloGene: 31141 GeneCards: TERT
Genska lokacija (miš)
Chromosome 13 (mouse)
Hr.Chromosome 13 (mouse)[1]
Chromosome 13 (mouse)
Genomska lokacija za TERT
Genomska lokacija za TERT
Band13 C1|13 40.12 cMStart73,775,030 bp[1]
Kraj73,797,962 bp[1]
Obrazac RNK izražavanja
More reference expression data
Genska ontologija
Molecular function• transferase activity
• DNA binding
• nucleotidyltransferase activity
• telomeric DNA binding
• tRNA binding
• metal ion binding
• RNA-directed 5'-3' RNA polymerase activity
• telomerase RNA reverse transcriptase activity
• GO:0001948, GO:0016582 везивање за протеине плазме
• transcription coactivator binding
• telomerase RNA binding
• telomerase activity
• protein homodimerization activity
• chaperone binding
• template-free RNA nucleotidyltransferase
• RNA binding
• protein C-terminus binding
• protein N-terminus binding
• identical protein binding
• Reverzna transkriptaza
• RNA-directed DNA polymerase activity
Cellular component• цитоплазма
• PML body
• nuclear telomere cap complex
• RNA-directed RNA polymerase complex
• Нуклеоплазма
• хромозом
• Теломера
• telomerase catalytic core complex
• Једарце
• митохондрије
• TERT-RMRP complex
• mitochondrial nucleoid
• једро
• ћелијска мембрана
• telomerase holoenzyme complex
• цитосол
• nuclear speck
Biological process• positive regulation of protein localization to nucleolus
• telomere maintenance via telomerase
• DNA biosynthetic process
• regulation of protein stability
• replicative senescence
• RNA-dependent DNA biosynthetic process
• mitochondrion organization
• negative regulation of gene expression
• transcription, RNA-templated
• positive regulation of Wnt signaling pathway
• GO:0070919 production of siRNA involved in RNA interference
• positive regulation of nitric-oxide synthase activity
• establishment of protein localization to telomere
• negative regulation of production of siRNA involved in RNA interference
• DNA strand elongation
• positive regulation of protein binding
• negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
• telomere maintenance
• GO:0062103 RNA biosynthetic process
• positive regulation of pri-miRNA transcription by RNA polymerase II
• negative regulation of cellular senescence
• response to cadmium ion
• positive regulation of G1/S transition of mitotic cell cycle
• beta-catenin-TCF complex assembly
• негативно регулисање апоптотичког процеса
• positive regulation of glucose import
• positive regulation of angiogenesis
• positive regulation of vascular associated smooth muscle cell migration
• negative regulation of glial cell proliferation
• positive regulation of transdifferentiation
• cellular response to hypoxia
• positive regulation of vascular associated smooth muscle cell proliferation
• negative regulation of endothelial cell apoptotic process
• GO:1904089 негативно уређење неурона процеса апоптозе
• positive regulation of hair cycle
• positive regulation of stem cell proliferation
Sources:Amigo / QuickGO
Ortolozi
VrsteČovekMiš
Entrez

7015

21752

Ensembl

ENSG00000164362

ENSMUSG00000021611

UniProt

O14746

O70372

RefSeq (mRNA)

NM_001193376
NM_198253
NM_198254
NM_198255

NM_009354
NM_001362387
NM_001362388

RefSeq (protein)

NP_001180305
NP_937983

NP_033380
NP_001349316
NP_001349317

Location (UCSC)n/aChr 13: 73.78 – 73.8 Mb
PubMed search[2][3]
Wikidata
View/Edit HumanView/Edit Mouse

Reverzna transkriptaza telomeraze (TERT, ili hTERT kod ljudi) je katalitička podjedinica enzima telomeraza, koja, zajedno sa telomeraznom RNK komponentom (TERC), sačinjava najvažniju jedinicu telomeraznog kompleksa.[4][5]

Telomeraze su deo distinktne podgrupe RNK-zavisnih polimeraza. Telomeraze produžavaju telomere u DNK lancima, čime omogućavaju starećim ćelijama koje bi postale postmitotičke i podlegle apoptozi da premaše Hejflikov limit i postanu potencijalno besmrtne, kao što je to često slučaj sa ćelijama raka. Specifično, TERT je odgovoran za katalizu adicije nukleotida u TTAGGG sequenci na krajevima hromozomskih telomera.[6] Ova adicija ponavljajućih DNK sekvenci spečava degradaciju krajeva hromozoma nakon višestrukih ciklusa replikacije.[7]

hTERT odstustvo (obično usled hromozomske mutacije) je vezano za sindrom mačjeg plača.[8][9]

Interakcije

Reverzna transkriptaza telomeraze formira interakcije sa:

Vidi još

Reference

  1. ^ а б в GRCm38: Ensembl release 89: ENSMUSG00000021611 - Ensembl, May 2017
  2. ^ „Human PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine. 
  3. ^ „Mouse PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine. 
  4. ^ Weinrich SL, Pruzan R, Ma L, Ouellette M, Tesmer VM, Holt SE, Bodnar AG, Lichtsteiner S, Kim NW, Trager JB, Taylor RD, Carlos R, Andrews WH, Wright WE, Shay JW, Harley CB, Morin GB (1997). „Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT”. Nat. Genet. 17 (4): 498—502. PMID 9398860. doi:10.1038/ng1297-498. 
  5. ^ Kirkpatrick KL, Mokbel K (2001). „The significance of human telomerase reverse transcriptase (hTERT) in cancer”. Eur J Surg Oncol. 27 (8): 754—60. PMID 11735173. doi:10.1053/ejso.2001.1151. 
  6. ^ Shampay J, Blackburn EH (1988). „Generation of telomere-length heterogeneity in Saccharomyces cerevisiae”. Proc. Natl. Acad. Sci. U.S.A. 85 (2): 534—8. PMC 279585 Слободан приступ. PMID 3277178. doi:10.1073/pnas.85.2.534. 
  7. ^ Poole JC, Andrews LG, Tollefsbol TO (2001). „Activity, function, and gene regulation of the catalytic subunit of telomerase (hTERT)”. Gene. 269 (1-2): 1—12. PMID 11376932. doi:10.1016/S0378-1119(01)00440-1. 
  8. ^ Zhang A, Zheng C, Hou M, Lindvall C, Li KJ, Erlandsson F, Björkholm M, Gruber A, Blennow E, Xu D (2003). „Deletion of the telomerase reverse transcriptase gene and haploinsufficiency of telomere maintenance in Cri du chat syndrome”. Am. J. Hum. Genet. 72 (4): 940—8. PMC 1180356 Слободан приступ. PMID 12629597. doi:10.1086/374565. 
  9. ^ Cerruti Mainardi P (2006). „Cri du Chat syndrome”. Orphanet J Rare Dis. 1: 33. PMC 1574300 Слободан приступ. PMID 16953888. doi:10.1186/1750-1172-1-33. 
  10. ^ Haendeler J, Hoffmann J, Rahman S, Zeiher AM, Dimmeler S (2003). „Regulation of telomerase activity and anti-apoptotic function by protein-protein interaction and phosphorylation”. FEBS Lett. 536 (1-3): 180—6. PMID 12586360. doi:10.1016/S0014-5793(03)00058-9. 
  11. ^ Kawauchi K, Ihjima K, Yamada O (2005). „IL-2 increases human telomerase reverse transcriptase activity transcriptionally and posttranslationally through phosphatidylinositol 3'-kinase/Akt, heat shock protein 90, and mammalian target of rapamycin in transformed NK cells”. J. Immunol. 174 (9): 5261—9. PMID 15843522. doi:10.4049/jimmunol.174.9.5261. 
  12. ^ а б Chai W, Ford LP, Lenertz L, Wright WE, Shay JW (2002). „Human Ku70/80 associates physically with telomerase through interaction with hTERT”. J. Biol. Chem. 277 (49): 47242—7. PMID 12377759. doi:10.1074/jbc.M208542200. 
  13. ^ Song H, Li Y, Chen G, Xing Z, Zhao J, Yokoyama KK, Li T, Zhao M (2004). „Human MCRS2, a cell-cycle-dependent protein, associates with LPTS/PinX1 and reduces the telomere length”. Biochem. Biophys. Res. Commun. 316 (4): 1116—23. PMID 15044100. doi:10.1016/j.bbrc.2004.02.166. 
  14. ^ Khurts S, Masutomi K, Delgermaa L, Arai K, Oishi N, Mizuno H, Hayashi N, Hahn WC, Murakami S (2004). „Nucleolin interacts with telomerase”. J. Biol. Chem. 279 (49): 51508—15. PMID 15371412. doi:10.1074/jbc.M407643200. 
  15. ^ Zhou XZ, Lu KP (2001). „The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor”. Cell. 107 (3): 347—59. PMID 11701125. doi:10.1016/S0092-8674(01)00538-4. 
  16. ^ Seimiya H, Sawada H, Muramatsu Y, Shimizu M, Ohko K, Yamane K, Tsuruo T (2000). „Involvement of 14-3-3 proteins in nuclear localization of telomerase”. EMBO J. 19 (11): 2652—61. PMC 212742 Слободан приступ. PMID 10835362. doi:10.1093/emboj/19.11.2652. 

Literatura

  • Mattson MP, Fu W, Zhang P (2001). „Emerging roles for telomerase in regulating cell differentiation and survival: a neuroscientist's perspective”. Mech. Ageing Dev. 122 (7): 659—71. PMID 11322991. doi:10.1016/S0047-6374(01)00221-4. 
  • Castillo Ureta H, Barrera Saldaña HA, Martínez Rodríguez HG (2003). „[Telomerase: an enzyme with multiple applications in cancer research]”. Rev. Invest. Clin. 54 (4): 342—8. PMID 12415959. 
  • Janknecht R (2004). „On the road to immortality: hTERT upregulation in cancer cells”. FEBS Lett. 564 (1–2): 9—13. PMID 15094035. doi:10.1016/S0014-5793(04)00356-4. 
  • Cristofari G, Sikora K, Lingner J (2007). „Telomerase unplugged”. ACS Chem. Biol. 2 (3): 155—8. PMID 17373762. doi:10.1021/cb700037c. 
  • Beliveau A, Yaswen P (2007). „Soothing the watchman: telomerase reduces the p53-dependent cellular stress response”. Cell Cycle. 6 (11): 1284—7. PMID 17534147. doi:10.4161/cc.6.11.4298. 
  • Bellon M, Nicot C (2007). „Telomerase: a crucial player in HTLV-I-induced human T-cell leukemia”. Cancer genomics & proteomics. 4 (1): 21—5. PMID 17726237. 

Spoljašnje veze

  • GeneReviews/NCBI/NIH/UW entry on Dyskeratosis Congenita
  • GeneReviews/NCBI/NIH/UW entry on Pulmonary Fibrosis, Familial
  • TERT+protein,+human на US National Library of Medicine Medical Subject Headings (MeSH)
  • п
  • р
  • у
Transferaze: fosfor-sadržavajuće grupe (EC 2.7)
2.7.1-2.7.4:
fosfotransferaze/kinaze
(PO4)
2.7.1: OH akceptor
2.7.2: COOH akceptor
2.7.3: N akceptor
2.7.4: PO4 akceptor
2.7.6: difosfotransferaze
(P2O7)2.7.7: nukleotidiltransferaza
(PO4-nukleozid)
Polimeraza
DNK polimeraza
  • DNK-usmerena DNK polimeraza: DNK polimeraza I
  • DNA polimeraza II
  • DNA polimeraza III holoenzim

RNK-usmerena DNA polimeraza: Reverzna transkriptaza (Telomeraza)

DNK nukleotidileksotransferaza/Terminalna deoksinukleotidil transferaza

RNK nukleotidiltransferaza
Fosforolitička
3' do 5' eksoribonukleaza
Uridililtransferaza
Guanililtransferaza
Druge
2.7.8: razno
Fosfatidiltransferaze
Glikozil-1-fosfotransferaza
  • N-acetilglukozamin-1-fosfat transferaza
2.7.10-2.7.13: proteinske kinaze
(PO4; proteinski akceptor)
2.7.10: proteinske-tirozinske
  • vidi tirozinske kinaze
2.7.11: proteinske-serin/treonin
  • vidi serin/treonin-specifične proteinske kinaze
2.7.12: dualna specifičnost
  • vidi serin/treonin-specifične proteinske kinaze
2.7.13: protein-histidinske
  • Protein-histidinske pros-kinaze
  • Proteinske-histidinske tele-kinaze
  • Histidinske kinaze
B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, 4.1/2/3/4/5/6, 5.1/2/3/4/99, 6.1-3/4/5-6
  • p
  • r
  • u
Separacija
i inicijacija
Prokariotska
(inicijacija)
Eukariotska
(priprema u
G1 fazi)
Oba
Replikacija
Prokariotska
(elongacija)
Eukariotska
(sinteza u
S fazi)
Oba
Kretanje: Procesivnost · DNK ligaza
Terminacija
B bsyn: dnk (repl, cycl, reco, repr) · tscr (fact, tcrg, nucl, rnat, rept, ptts) · tltn (risu, pttl, nexn) · dnab, rnab/runp · stru (domn, 1°, 2°, 3°, 4°)
Нормативна контрола Уреди на Википодацима
  • Енциклопедија Британика